Preliminary Report on Dengue fever in patients from
Dengue fever in patients coming from
In March 2013, 8 patients were diagnosed [with dengue virus infections], 7 of them in the last 2 weeks of this month [April 2013]; 11 additional patients were diagnosed so far in April 2013. Ages ranged from 22-67 years; 3 patients were female. Patients were residents or travelers to the
Laboratory confirmation of the clinical diagnosis was established at the Tropical Clinic Unit Laboratory of the Instituto de Higiene e Medicina Tropical (IHMT,
Samples from 4 potentially viremic patients were selected to detect dengue virus (DENV) using RT-PCR at the Virology Laboratory of the Medical Microbiology Unit of the IHMT. The 1st PCR round was performed according to Lanciotti et al (1992, J.Clin.Micro., vol.30, n3, pp: 545-551), with adaptations from Harris et al (1998, J.Clin.Micro., vol.36, (9):2634-2639). The 2nd PCR round was a multiplex designed to serotype DENV according to the amplification product obtained (482bp-DENV1; 119bp-DENV2; 290bp-DENV3; 392bp-DENV4). In the 4 samples, amplification detected product compatible with RNA from DENV1. Sequencing and viral culture are ongoing.
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ProMED receives relatively few reports of dengue virus infections in
It is becoming increasingly clear that dengue fever virus infections in
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